光电控制补能的短线傅科摆

给出短线傅科摆的一种新设计，它采用无触点的光电控制补能系统而不是有触点的“ｃｈａｒｒｏｎ ｒｉｎｇ”。     

用CCD分析光源的辐射光谱

本文介绍了利用光电图象技术对光源光谱进行分析的工作。利用ＣＣＤ线阵器件和计算机，我们获得了黑体、灰体及单色光源的光谱特性以及有关数据。     

Specific Detection of Salmonella typhi Using Renewable Amperometric Immunosensor

A renewable amperometric immunosensor was developed for the specific detection of Salmonella typhi (S. typhi) using flagellin specific antibodies. An immunocomposite comprising paraffin, graphite, and capturing antibodies against S. typhi was used to construct the electrode. The detection technique involved a sandwich ELISA system. The assay conditions were optimized for loading of capturing antibody, incubation time for S. typhi cells, rotation speed and minimum amount of substrate needed. 1‐naphthyl phosphate was used as the substrate with an amperometric detection of its enzymatic hydrolysis product 1‐naphthol at a potential of +400 mV vs Ag/AgCl reference electrode. The minimum detection limit for S. typhi was found to be 10⁵ cells/mL in 90 min, while ELISA detects 10⁶ cells/mL in five hours.     

Quantum chaology: The photoeffect and beyond

I will show how aspects of quantum chaology are relevant even in a seemingly well understood quantum phenomenon like the photoelectric effect. This example together with recent experiments in atom optics are used to define and discuss the larger questions, recent progress made by us in resolving some of these issues and future directions.     

偏振光的特性研究及其检测综合实验简介

利用微机辅助开设了偏振光特性研究和检测实验，该实验使光学实验与电子线路、微机原理及接口技术相结合，可作为对高年级学生开设的综合性实验。     

Love波免疫传感器在免疫分析中的研究

Love波免疫传感器是一种以免疫反应为识别方式的新型声表面波传感器。与其它压电声波免疫传感技术相比,水平剪切型表面波、高的压电晶体基频以及声波导层的存在使Love波免疫传感器在可用于气液两相检测的同时具有更高的灵敏度,从而可以成为免疫分析中的一种重要的工具。本文分别从Love波传感器的构造、原理、发展现状和以抗体作为探针在传感器表面的固定化方法两方面综述了Love波免疫传感器的研究进展。     

Evaluation of a glucose sensing antibody using weak affinity chromatography

Continuous monitoring of drug levels and endogenous molecules in biological fluids is a developing research area with many applications. One example is the need to improve life for millions of diabetes mellitus patients by continuously monitoring the glucose level. In order to have a dynamic response, the recognition molecule in a continuous sensor should preferentially have a fast dissociation rate and a dissociation constant in the millimolar range. We have evaluated the monoclonal antibody (mAb) 3F1E8-A2 for its potential to be used in a future glucose sensor application. The mAb was generated from hybridomas by immunizing mice with 10 kDa dextran (an alpha1,6-glucose polymer) with the aim of obtaining mAbs that can recognize the glucose monomer. The mAb was immobilized to macroporous silica and the interaction with dextran-derived oligosaccharides was evaluated with weak affinity chromatography (WAC). To measure the low affinities between the mAb 3F1E8-A2 and different monosaccharides, a competitive weak affinity chromatography approach was employed. It was found that the mAb had a higher specificity for glucose compared with other monosaccharides and the dissociation constant (K(d)) towards glucose was determined as 18.8 +/- 2.6 mm.     

Immunosensor for the determination of <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> using a tyrosinase–mercaptopropionic acid modified electrode as an amperometric transducer

An amperometric immunosensor for the quantification of Staphylococcus aureus based on the coimmobilization of rabbit immunoglobulin G (RbIgG) and tyrosinase on a mercaptopropionic acid self-assembled monolayer modified gold electrode is reported. A competitive mode in which protein-A-bearing S. aureus cells and antiRbIgG labeled with alkaline phosphatase (AP) compete for the binding sites of immobilized RbIgG was used. Monitoring of the affinity reaction was carried out by the amperometric detection at -0.15 V of phenol generated in the enzyme reaction with AP, at the tyrosinase-modified electrode through the electrochemical reduction of the o-quinone formed. Optimization of the working variables, such as the immunosensor composition and incubation times, the applied potential, the working pH and the concentration of phenyl phosphate used as the AP substrate, was carried out. Under the optimized conditions, both the repeatability of the measurements and the reproducibility of the responses obtained with different immunosensors yielded relative standard deviation values for the steady-state current lower than 10%. The immunosensor showed a dynamic range from 4.4x10(5) to 1.8x10(7) S. aureus cells mL(-1), with a detection limit of 1.7x10(5) cells mL(-1). The limit of detection was remarkably improved by subjecting S. aureus cells to wall lysis by heat treatment. The value obtained was 2.3x10(3) cells mL(-1), which is adequate for the monitoring of S. aureus contamination levels in some foodstuffs. As an application, milk samples spiked with bacteria at the 4.8x10(3) cells mL(-1) level were analyzed.     

Labelless impedance immunosensor based on polypyrrole-pyrolecarboxylic acid copolymer for hCG detection

In this work, a sensitive label-free impedimetric hCG-immunosensor was constructed by using a commercial screen-printing carbon ink electrode (namely disposable electrochemical printed chip) as the basis. The carbon ink electrode of DEP chip is modified first by deposition of polypyrrole-pyrole-2-carboxylic acid copolymer and thence hCG antibody immobilization via the COOH groups of pyrrole-2-carboxylic acid, which can serve as a linker for covalent biomolecular immobilization. The experimental results exposed that the designed immunosensor is more sensitive than other previously reported immunosensors, in the case of detection limit and linear range for antigen detection. With optimal fabrication parameters, the detection limit for α-hCG was 2.3 pg/mL in 10 mM phosphate buffer saline (PBS) solution containing 1% bovine serum albumine (BSA). Moreover, the use of inexpensive DEP chip as a basis for these immunosensors will allow for simple instrumentation, disposable and portable at low cost. This work also demonstrates a new approach to develop a sensitive and label-free impedimetric immunosensor based on screen-printed electrode for applications in clinical diagnosis.     

Composite Electrode SnO2／TiO2 for Dye-Sensitized Solar Cells

Composite nanoporous electrode SnO2/TiO2 was fabricated for the dye sensitized solar cell (DSSC) with N3 (Cis-Ru). After introducing of TiO2, the open-circuit photovoltage (Voc) was higher than that of the pure SnO2 electrode, while short-circuit photocurrent (Isc) was varied with the ratio of the TiO2. Appropriate content of the TiO2 can be beneficial to the efficiency of the solar cell, and it gives negative impact on the composite electrode when the content of TiO2 is higher.